|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SPINK4 ORF mammalian expression plasmid, C-GFPSpark tag||HG11669-ACG|
|Human SPINK4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11669-ACR|
|Human SPINK4 ORF mammalian expression plasmid, C-Flag tag||HG11669-CF|
|Human SPINK4 ORF mammalian expression plasmid, C-His tag||HG11669-CH|
|Human SPINK4 ORF mammalian expression plasmid, C-Myc tag||HG11669-CM|
|Human SPINK4 ORF mammalian expression plasmid, C-HA tag||HG11669-CY|
|Human SPINK4 Gene cDNA clone plasmid||HG11669-M|
|Human SPINK4 ORF mammalian expression plasmid, N-Flag tag||HG11669-NF|
|Human SPINK4 ORF mammalian expression plasmid, N-His tag||HG11669-NH|
|Human SPINK4 ORF mammalian expression plasmid, N-Myc tag||HG11669-NM|
|Human SPINK4 ORF mammalian expression plasmid, N-HA tag||HG11669-NY|
|Human SPINK4 natural ORF mammalian expression plasmid||HG11669-UT|
|Learn more about expression Vectors|
Serine protease inhibitor Kazal-type 4, also known as Peptide PEC-60 homolog and SPINK4, is a secreted protein which contains one Kazal-like domain. SPINK4 is a member of the SPINK protein family. The gene family of serine protease inhibitors of the Kazal type (SPINK) are functional and positional candidate genes for celiac disease (CD). SPINK1 plays an important role in protecting the pancreas against excessive trypsinogen activation. It is a potent natural inhibitor of pancreatic trypsin activity. SPINK1 mutations are associated with the development of acute and chronic pancreatitis and have been detected in all forms of chronic pancreatitis. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. The SPINK2 protein contains a typical Kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK9 was identified in human skin. Its expression was strong in palmar epidermis, but not detectable or very low in non palmoplantar skin.