|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FAM3D ORF mammalian expression plasmid, C-GFPSpark tag||HG11659-ACG|
|Human FAM3D ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11659-ACR|
|Human FAM3D ORF mammalian expression plasmid, C-Flag tag||HG11659-CF|
|Human FAM3D ORF mammalian expression plasmid, C-His tag||HG11659-CH|
|Human FAM3D ORF mammalian expression plasmid, C-Myc tag||HG11659-CM|
|Human FAM3D ORF mammalian expression plasmid, C-HA tag||HG11659-CY|
|人 FAM3D 基因全长ORF克隆||HG11659-M|
|Human FAM3D ORF mammalian expression plasmid, N-Flag tag||HG11659-NF|
|Human FAM3D ORF mammalian expression plasmid, N-His tag||HG11659-NH|
|Human FAM3D ORF mammalian expression plasmid, N-Myc tag||HG11659-NM|
|Human FAM3D ORF mammalian expression plasmid, N-HA tag||HG11659-NY|
|Human FAM3D natural ORF mammalian expression plasmid||HG11659-UT|
|Learn more about expression Vectors|
Family with sequence similarity 3 (FAM3) family is a novel cytokine-like gene family, which has four genes in this family, FAM3A, FAM3B, FAM3C, and FAM3D, each encoding a protein (224-235 amino acids) with a hydrophobic leader sequence. It had indicated that FAM3B/PANDER (pancreatic derived factor) is highly expressed in pancreas, and FAM3A and FAM3C in almost all tissues. FAM3D is abundantly expressed in placenta and weakly expressed in small intestine. Immunohistochemistry showed that FAM3A is expressed prominently in the vascular endothelium, particularly capillaries. FAM3A and FAM3B protein were both localized to the islets of Langerhans of the endocrine pancreas. Recombinant FAM3B protein has delayed effects on beta-cell function. FAM3C is involved in retinal laminar formation processes in vertebrates. NFATC2, SCP2, CACNA1C, TCRA, POLE, and FAM3D, were associated with narcolepsy. Some of these associations were further supported by gene expression analyses and an association study in essential hypersomnia (EHS), CNS hypersonia similar to narcolepsy.