|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SULT1A1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11658-ACG|
|Human SULT1A1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11658-ACR|
|Human SULT1A1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11658-ANG|
|Human SULT1A1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11658-ANR|
|Human SULT1A1 ORF mammalian expression plasmid, C-Flag tag||HG11658-CF|
|Human SULT1A1 ORF mammalian expression plasmid, C-His tag||HG11658-CH|
|Human SULT1A1 ORF mammalian expression plasmid, C-Myc tag||HG11658-CM|
|Human SULT1A1 ORF mammalian expression plasmid, C-HA tag||HG11658-CY|
|Human SULT1A1 Gene cDNA clone plasmid||HG11658-M|
|Human SULT1A1 ORF mammalian expression plasmid, N-Flag tag||HG11658-NF|
|Human SULT1A1 ORF mammalian expression plasmid, N-His tag||HG11658-NH|
|Human SULT1A1 ORF mammalian expression plasmid, N-Myc tag||HG11658-NM|
|Human SULT1A1 ORF mammalian expression plasmid, N-HA tag||HG11658-NY|
|Human SULT1A1 natural ORF mammalian expression plasmid||HG11658-UT|
|Learn more about expression Vectors|
Sulfate conjugation catalyzed by cytosolic sulfotransferase (SULT) enzymes. The SULTs are Phase II drug-metabolizing enzymes that catalyze the addition of a sulfuryl moiety to both endogenous compounds, including steroids and neurotransmitters, and certain xenobiotics, including N-hydroxy-2-acetylaminoflourine and phenolic compounds, like alpha-naphthol. SULTs may be involved in the individual genetic disposition, species differences, and organotropisms for toxicological effects of chemicals. Particularly SULT1A1 (Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1), a member of the sulfotransferase 1 subfamily, which is a major pathway for drug metabolism in humans. Humans have at least 10 functional SULT genes. There has been an explosion in information on sulfotransferase polymorphisms and their functional consequences. An Arg213His polymorphism in SULT1A1 has a strong influence on the level of enzyme protein and activity in platelets, which have been widely used for phenotyping. Statistically significant associations were observed between the SULT1A1 genotype (Arg213His) and age, obesity and certain neoplasias (mammary, pulmonary, esophageal and urothelial cancer). Furthermore, the polymorphism of the SULT1A1 may be closely associated with breast cancer.