|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ENPP5 ORF mammalian expression plasmid, C-GFPSpark tag||HG11519-ACG|
|Human ENPP5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11519-ACR|
|Human ENPP5 ORF mammalian expression plasmid, C-Flag tag||HG11519-CF|
|Human ENPP5 ORF mammalian expression plasmid, C-His tag||HG11519-CH|
|Human ENPP5 ORF mammalian expression plasmid, C-Myc tag||HG11519-CM|
|Human ENPP5 ORF mammalian expression plasmid, C-HA tag||HG11519-CY|
|Human ENPP5 Gene cDNA clone plasmid||HG11519-M|
|Human ENPP5 ORF mammalian expression plasmid, Flag tag||HG11519-M-F|
|Human ENPP5 ORF mammalian expression plasmid, N-Flag tag||HG11519-NF|
|Human ENPP5 ORF mammalian expression plasmid, N-His tag||HG11519-NH|
|Human ENPP5 ORF mammalian expression plasmid, N-Myc tag||HG11519-NM|
|Human ENPP5 ORF mammalian expression plasmid, N-HA tag||HG11519-NY|
|Human ENPP5 natural ORF mammalian expression plasmid||HG11519-UT|
|Learn more about expression Vectors|
ENPP5 is a member of the nucleotide pyrophosphatase/phosphodiesterase family(NPP). It is a family comprised by dimeric enzymes that catalyze the hydrolysis of phosphate diester bonds. There are seven isoforms in NPP family, some of which prefer nucleotide substrates, some of which prefer phospholipid substrates, and others of which prefer substrates that have not yet been determined. NPP also belongs to the alkaline phosphatase (AP) superfamily of enzymes and they are located in the cell membrane and hydrolyze extracellular phosphate diesters to affect a wide variety of biological processes. ENPP5 belongs to a group of nucleotidemetabolizing ectoenzymes, which regulate the availability of extracellular nucleotides. ENPP5 may play a role in neuronal cell communication. Hhowever, it lacks nucleotide pyrophosphatase and lysopholipase D activity. It may also be involved in neuronal cell communication. The amino acid sequence of human ENPP5 is 100%, 88%, and 82% identical to that of chimpanzee, dog and mouse/rat. ENPP5 functions in phospholipid metabolism.