|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human BNIPL ORF mammalian expression plasmid, C-GFPSpark tag||HG11460-ACG|
|Human BNIPL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11460-ACR|
|Human BNIPL ORF mammalian expression plasmid, N-GFPSpark tag||HG11460-ANG|
|Human BNIPL ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11460-ANR|
|Human BNIPL ORF mammalian expression plasmid, C-Flag tag||HG11460-CF|
|Human BNIPL ORF mammalian expression plasmid, C-His tag||HG11460-CH|
|Human BNIPL ORF mammalian expression plasmid, C-Myc tag||HG11460-CM|
|Human BNIPL ORF mammalian expression plasmid, C-HA tag||HG11460-CY|
|Human BNIPL Gene cDNA clone plasmid||HG11460-M|
|Human BNIPL ORF mammalian expression plasmid, N-Flag tag||HG11460-NF|
|Human BNIPL ORF mammalian expression plasmid, N-His tag||HG11460-NH|
|Human BNIPL ORF mammalian expression plasmid, N-Myc tag||HG11460-NM|
|Human BNIPL ORF mammalian expression plasmid, N-HA tag||HG11460-NY|
|Human BNIPL natural ORF mammalian expression plasmid||HG11460-UT|
|Learn more about expression Vectors|
BNIP-2- like (BNIPL), also known as Bcl-2/adenovirus E1B 19 kDa interacting protein 2-like, is a apaptosis-associated protein that shares 72% homology with BNIP-2. BNIPL is highly expressed in human placenta and lung. There are two alternative splices: BNIPL-1 and BNIPL-2. Both BNIPL-1 and BNIPL-2 have a conserved arginine-patch motif in Rho protein family. BNIPL-1 has function in suppressing cell growth through binding to MIF (macrophage migration inhibitory factor) and GFER (growth factor erv1(saccharomyces cerevisiae)-like). BNIPL-2 may suppress the Cdc42GAP’s activity through ineracting with Cdc42GAP. BNIPL-2, containg a key BCH domain, which may induce the cell pseudopod growth, takes part in modulating cell migrations. Thus it suggested BNIPL-2 implicated in cell migration progresses and tumor translocation.