|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine ACVR1B ORF mammalian expression plasmid, C-GFPSpark tag||DG70061-ACG|
|Canine ACVR1B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70061-ACR|
|Canine ACVR1B ORF mammalian expression plasmid, C-Flag tag||DG70061-CF|
|Canine ACVR1B ORF mammalian expression plasmid, C-His tag||DG70061-CH|
|Canine ACVR1B ORF mammalian expression plasmid, C-Myc tag||DG70061-CM|
|Canine ACVR1B ORF mammalian expression plasmid, C-HA tag||DG70061-CY|
|Canine ACVR1B Gene cDNA clone plasmid||DG70061-G|
|Canine ACVR1B ORF mammalian expression plasmid, N-Flag tag||DG70061-NF|
|Canine ACVR1B ORF mammalian expression plasmid, N-His tag||DG70061-NH|
|Canine ACVR1B ORF mammalian expression plasmid, N-Myc tag||DG70061-NM|
|Canine ACVR1B ORF mammalian expression plasmid, N-HA tag||DG70061-NY|
|Canine ACVR1B natural ORF mammalian expression plasmid||DG70061-UT|
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ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination.