|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine FGF18 ORF mammalian expression plasmid, C-GFPSpark tag||DG70059-ACG|
|Canine FGF18 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70059-ACR|
|Canine FGF18 ORF mammalian expression plasmid, C-Flag tag||DG70059-CF|
|Canine FGF18 ORF mammalian expression plasmid, C-His tag||DG70059-CH|
|Canine FGF18 ORF mammalian expression plasmid, C-Myc tag||DG70059-CM|
|Canine FGF18 ORF mammalian expression plasmid, C-HA tag||DG70059-CY|
|Canine FGF18 Gene cDNA clone plasmid||DG70059-G|
|Canine FGF18 ORF mammalian expression plasmid, N-Flag tag||DG70059-NF|
|Canine FGF18 ORF mammalian expression plasmid, N-His tag||DG70059-NH|
|Canine FGF18 ORF mammalian expression plasmid, N-Myc tag||DG70059-NM|
|Canine FGF18 ORF mammalian expression plasmid, N-HA tag||DG70059-NY|
|Canine FGF18 natural ORF mammalian expression plasmid||DG70059-UT|
|Learn more about expression Vectors|
Fibroblast growth factor 18 (FGF18) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that FGF18 is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Experiment datas identified FGF18 as a selective ligand for FGFR3 in limb bud mesenchymal cells, which suppressed proliferation and promoted their differentiation and production of cartilage matrix. FGF18 appears to regulate cell proliferation and differentiation positively in osteogenesis and negatively in chondrogenesis.