|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine FGF1 ORF mammalian expression plasmid, C-Flag tag||DG70039-CF|
|Canine FGF1 ORF mammalian expression plasmid, C-His tag||DG70039-CH|
|Canine FGF1 ORF mammalian expression plasmid, C-Myc tag||DG70039-CM|
|Canine FGF1 ORF mammalian expression plasmid, C-HA tag||DG70039-CY|
|Canine FGF1 ORF mammalian expression plasmid, N-Flag tag||DG70039-NF|
|Canine FGF1 ORF mammalian expression plasmid, N-His tag||DG70039-NH|
|Canine FGF1 ORF mammalian expression plasmid, N-Myc tag||DG70039-NM|
|Canine FGF1 ORF mammalian expression plasmid, N-HA tag||DG70039-NY|
|Canine FGF1 natural ORF mammalian expression plasmid||DG70039-UT|
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aFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1–4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.