|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SULT2A1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11411-ACG|
|Human SULT2A1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11411-ACR|
|Human SULT2A1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11411-ANG|
|Human SULT2A1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11411-ANR|
|Human SULT2A1 ORF mammalian expression plasmid, C-Flag tag||HG11411-CF|
|Human SULT2A1 ORF mammalian expression plasmid, C-His tag||HG11411-CH|
|Human SULT2A1 ORF mammalian expression plasmid, C-Myc tag||HG11411-CM|
|Human SULT2A1 ORF mammalian expression plasmid, C-HA tag||HG11411-CY|
|Human SULT2A1 Gene cDNA clone plasmid||HG11411-M|
|Human SULT2A1 natural ORF mammalian expression plasmid||HG11411-M-N|
|Human SULT2A1 ORF mammalian expression plasmid, N-Flag tag||HG11411-NF|
|Human SULT2A1 ORF mammalian expression plasmid, N-His tag||HG11411-NH|
|Human SULT2A1 ORF mammalian expression plasmid, N-Myc tag||HG11411-NM|
|Human SULT2A1 ORF mammalian expression plasmid, N-HA tag||HG11411-NY|
|Human SULT2A1 natural ORF mammalian expression plasmid||HG11411-UT|
|Learn more about expression Vectors|
Hydroxysteroid sulfotransferase ( SULT2A1 ) is a key enzyme in the testicular and hepatic metabolism of 5alpha-androstenone, which is a major component of the off-odor and off-flavor in pork known as boar taint. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. SULT2A1 is a sulfo-conjugating phase II enzyme expressed at very high levels in the liver and intestine, the two major first-pass metabolic tissues, and in the steroidogenic adrenal tissue. SULT2A1 acts preferentially on the hydroxysteroids dehydroepiandrosterone, testosterone/dihydrotestosterone, and pregnenolone and on cholesterol-derived amphipathic sterol bile acids.