|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine IL5 ORF mammalian expression plasmid, C-GFPSpark tag||DG70019-ACG|
|Canine IL5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70019-ACR|
|Canine IL5 ORF mammalian expression plasmid, C-Flag tag||DG70019-CF|
|Canine IL5 ORF mammalian expression plasmid, C-His tag||DG70019-CH|
|Canine IL5 ORF mammalian expression plasmid, C-Myc tag||DG70019-CM|
|Canine IL5 ORF mammalian expression plasmid, C-HA tag||DG70019-CY|
|Canine IL5 Gene cDNA clone plasmid||DG70019-G|
|Canine IL5 ORF mammalian expression plasmid, N-Flag tag||DG70019-NF|
|Canine IL5 ORF mammalian expression plasmid, N-His tag||DG70019-NH|
|Canine IL5 ORF mammalian expression plasmid, N-Myc tag||DG70019-NM|
|Canine IL5 ORF mammalian expression plasmid, N-HA tag||DG70019-NY|
|Canine IL5 natural ORF mammalian expression plasmid||DG70019-UT|
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Interleukin 5 (IL-5) is a member of the interleukin family with length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins / signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered as a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues, and can modulate eosinophil behavior at every stage from maturation to survival.