|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine IL1B ORF mammalian expression plasmid, C-GFPSpark tag||DG70018-ACG|
|Canine IL1B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70018-ACR|
|Canine IL1B ORF mammalian expression plasmid, C-Flag tag||DG70018-CF|
|Canine IL1B ORF mammalian expression plasmid, C-His tag||DG70018-CH|
|Canine IL1B ORF mammalian expression plasmid, C-Myc tag||DG70018-CM|
|Canine IL1B ORF mammalian expression plasmid, C-HA tag||DG70018-CY|
|Canine IL1B Gene cDNA clone plasmid||DG70018-G|
|Canine IL1B ORF mammalian expression plasmid, N-Flag tag||DG70018-NF|
|Canine IL1B ORF mammalian expression plasmid, N-His tag||DG70018-NH|
|Canine IL1B ORF mammalian expression plasmid, N-Myc tag||DG70018-NM|
|Canine IL1B ORF mammalian expression plasmid, N-HA tag||DG70018-NY|
|Canine IL1B natural ORF mammalian expression plasmid||DG70018-UT|
|Learn more about expression Vectors|
Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).