|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD53 ORF mammalian expression plasmid, C-GFPSpark tag||HG11409-ACG|
|Human CD53 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11409-ACR|
|Human CD53 ORF mammalian expression plasmid, C-Flag tag||HG11409-CF|
|Human CD53 ORF mammalian expression plasmid, C-His tag||HG11409-CH|
|Human CD53 ORF mammalian expression plasmid, C-Myc tag||HG11409-CM|
|Human CD53 ORF mammalian expression plasmid, C-HA tag||HG11409-CY|
|Human CD53 Gene cDNA clone plasmid||HG11409-M|
|Human CD53 ORF mammalian expression plasmid, N-Flag tag||HG11409-NF|
|Human CD53 ORF mammalian expression plasmid, N-His tag||HG11409-NH|
|Human CD53 ORF mammalian expression plasmid, N-Myc tag||HG11409-NM|
|Human CD53 ORF mammalian expression plasmid, N-HA tag||HG11409-NY|
|Human CD53 natural ORF mammalian expression plasmid||HG11409-UT|
|Learn more about expression Vectors|
CD53 is a member of the transmembrane 4 superfamily, also called the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. Familial deficiency of CD53 gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation.