|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SULT1A3 ORF mammalian expression plasmid, C-GFPSpark tag||HG11408-ACG|
|Human SULT1A3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11408-ACR|
|Human SULT1A3 ORF mammalian expression plasmid, N-GFPSpark tag||HG11408-ANG|
|Human SULT1A3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11408-ANR|
|Human SULT1A3 ORF mammalian expression plasmid, C-Flag tag||HG11408-CF|
|Human SULT1A3 ORF mammalian expression plasmid, C-His tag||HG11408-CH|
|Human SULT1A3 ORF mammalian expression plasmid, C-Myc tag||HG11408-CM|
|Human SULT1A3 ORF mammalian expression plasmid, C-HA tag||HG11408-CY|
|Human SULT1A3 Gene cDNA clone plasmid||HG11408-M|
|Human SULT1A3 ORF mammalian expression plasmid, N-Flag tag||HG11408-NF|
|Human SULT1A3 ORF mammalian expression plasmid, N-His tag||HG11408-NH|
|Human SULT1A3 ORF mammalian expression plasmid, N-Myc tag||HG11408-NM|
|Human SULT1A3 ORF mammalian expression plasmid, N-HA tag||HG11408-NY|
|Human SULT1A3 natural ORF mammalian expression plasmid||HG11408-UT|
|Learn more about expression Vectors|
SULT1A3 belongs to the sulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are different in their tissue distributions and substrate specificities while their gene structure (number and length of exons) is similar. SULT1A3 gene encodes a phenol sulfotransferase with thermolabile enzyme activity. Four sulfotransferase genes are located on the p arm of chromosome 16; this gene and SULT1A4 arose from a segmental duplication. It is the most centromeric of the four sulfotransferase genes. Exons of this gene overlap with exons of a gene that encodes a protein containing GIY-YIG domains (GIYD1). SULT1A3 is expressed in liver, colon, kidney, lung, brain, spleen, small intestine, placenta and leukocyte. SULT1A3 is a sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.