|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human NMNAT2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11399-ACG|
|Human NMNAT2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11399-ACR|
|Human NMNAT2 ORF mammalian expression plasmid, N-GFPSpark tag||HG11399-ANG|
|Human NMNAT2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11399-ANR|
|Human NMNAT2 ORF mammalian expression plasmid, C-Flag tag||HG11399-CF|
|Human NMNAT2 ORF mammalian expression plasmid, C-His tag||HG11399-CH|
|Human NMNAT2 ORF mammalian expression plasmid, C-Myc tag||HG11399-CM|
|Human NMNAT2 ORF mammalian expression plasmid, C-HA tag||HG11399-CY|
|Human NMNAT2 Gene cDNA clone plasmid||HG11399-M|
|Human NMNAT2 ORF mammalian expression plasmid, N-Flag tag||HG11399-NF|
|Human NMNAT2 ORF mammalian expression plasmid, N-His tag||HG11399-NH|
|Human NMNAT2 ORF mammalian expression plasmid, N-Myc tag||HG11399-NM|
|Human NMNAT2 ORF mammalian expression plasmid, N-HA tag||HG11399-NY|
|Human NMNAT2 natural ORF mammalian expression plasmid||HG11399-UT|
|Learn more about expression Vectors|
NMNAT2, also known as NMNAT-2, belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family. NMNAT is a central enzyme in NAD+ biosynthesis, transferring the adenylyl moiety of ATP to nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) resulting in the formation of NAD+ or NaAD+ and the release of pyrophosphate. NMNAT2 is predominantly expressed in human pancreas, insulinoma as well as in the brain, especially in the cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Immunofluorescence microscopy localized endogenous NMNAT2 to the Golgi apparatus in human cell line. Endogenous NMNAT2 seem to be a labile axon survival factor, because specific depletion of NMNAT2 is sufficient to induce Wallerian-like degeneration of uninjured axons which endogenous NMNAT1 and NMNAT3 cannot prevent. Thus endogenous NMNAT2 represents an exciting new therapeutic target for axonal disorders.