|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine CAR9 ORF mammalian expression plasmid, C-GFPSpark tag||DG70002-ACG|
|Canine CAR9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70002-ACR|
|Canine CAR9 ORF mammalian expression plasmid, C-Flag tag||DG70002-CF|
|Canine CAR9 ORF mammalian expression plasmid, C-His tag||DG70002-CH|
|Canine CAR9 ORF mammalian expression plasmid, C-Myc tag||DG70002-CM|
|Canine CAR9 ORF mammalian expression plasmid, C-HA tag||DG70002-CY|
|Canine CAR9 Gene cDNA clone plasmid||DG70002-G|
|Canine CAR9 ORF mammalian expression plasmid, N-Flag tag||DG70002-NF|
|Canine CAR9 ORF mammalian expression plasmid, N-His tag||DG70002-NH|
|Canine CAR9 ORF mammalian expression plasmid, N-Myc tag||DG70002-NM|
|Canine CAR9 ORF mammalian expression plasmid, N-HA tag||DG70002-NY|
|Canine CAR9 natural ORF mammalian expression plasmid||DG70002-UT|
|Learn more about expression Vectors|
Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.