|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus H1F0 ORF mammalian expression plasmid, C-GFPSpark tag||CG90787-ACG|
|Rhesus H1F0 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90787-ACR|
|Rhesus H1F0 ORF mammalian expression plasmid, N-GFPSpark tag||CG90787-ANG|
|Rhesus H1F0 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||CG90787-ANR|
|Rhesus H1F0 ORF mammalian expression plasmid, C-Flag tag||CG90787-CF|
|Rhesus H1F0 ORF mammalian expression plasmid, C-His tag||CG90787-CH|
|Rhesus H1F0 ORF mammalian expression plasmid, C-Myc tag||CG90787-CM|
|Rhesus H1F0 ORF mammalian expression plasmid, C-HA tag||CG90787-CY|
|Rhesus H1F0 Gene cDNA clone plasmid||CG90787-G|
|Rhesus H1F0 ORF mammalian expression plasmid, N-Flag tag||CG90787-NF|
|Rhesus H1F0 ORF mammalian expression plasmid, N-His tag||CG90787-NH|
|Rhesus H1F0 ORF mammalian expression plasmid, N-Myc tag||CG90787-NM|
|Rhesus H1F0 ORF mammalian expression plasmid, N-HA tag||CG90787-NY|
|Rhesus H1F0 natural ORF mammalian expression plasmid||CG90787-UT|
|Learn more about expression Vectors|
H1 histone family, member 0 (H1F0) is a member of the H1 histone family of nuclear proteins which are a component of chromatin in eukaryotic cells. It's involved in maintaining the structure of chromatin by packing the "beads on a string" sub-structure into a high order structure. The lysine-rich H1 histone family in mammals includes eleven members. In higher eukaryotes all H1 variants have the same general structure, consisting of a central conserved globular domain and less conserved N-terminal and C-terminal tails. These tails are moderately conserved among species, but differ among variants, suggesting a specific function for each H1 variant. Studies on the role of particular subtypes at specific developmental stages in lower eukaryotes, but also in vertebrates suggest that specific subtypes of H1 participate in particular systems of gene regulation.