|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MGAT5 ORF mammalian expression plasmid, C-GFPSpark tag||HG11373-ACG|
|Human MGAT5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11373-ACR|
|Human MGAT5 ORF mammalian expression plasmid, C-Flag tag||HG11373-CF|
|Human MGAT5 ORF mammalian expression plasmid, C-His tag||HG11373-CH|
|Human MGAT5 ORF mammalian expression plasmid, C-Myc tag||HG11373-CM|
|Human MGAT5 ORF mammalian expression plasmid, C-HA tag||HG11373-CY|
|Human MGAT5 Gene cDNA clone plasmid||HG11373-M|
|Human MGAT5 ORF mammalian expression plasmid, N-Flag tag||HG11373-NF|
|Human MGAT5 ORF mammalian expression plasmid, N-His tag||HG11373-NH|
|Human MGAT5 ORF mammalian expression plasmid, N-Myc tag||HG11373-NM|
|Human MGAT5 ORF mammalian expression plasmid, N-HA tag||HG11373-NY|
|Human MGAT5 natural ORF mammalian expression plasmid||HG11373-UT|
|Learn more about expression Vectors|
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A, also known as Alpha-mannoside beta-1,6-N-acetylglucosaminyl-transferase, Mannoside acetylglucosaminyltransferase 5, N-acetylglucosaminyl-transferase V, MGAT5 and GGNT5, is a single-pass type I I membrane protein which belongs to the glycosyltransferase 18 family. MGAT5 / GGNT5 catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. The central nervous system (CNS) is rich in glycoconjugates, located on cell surface and in extracellular matrix. MGAT5 / GGNT5 modification of complex-type N-glycans on CNS glycoproteins is involved in the regulation of depression-like behavior. Inhibitors of MGAT5 / GGNT5 might be useful in the treatment of malignancies by targeting their dependency on focal adhesion signaling for growth and metastasis.