|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ADSL ORF mammalian expression plasmid, C-GFPSpark tag||HG11287-ACG|
|Human ADSL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11287-ACR|
|Human ADSL ORF mammalian expression plasmid, N-GFPSpark tag||HG11287-ANG|
|Human ADSL ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11287-ANR|
|Human ADSL ORF mammalian expression plasmid, C-Flag tag||HG11287-CF|
|Human ADSL ORF mammalian expression plasmid, C-His tag||HG11287-CH|
|Human ADSL ORF mammalian expression plasmid, C-Myc tag||HG11287-CM|
|Human ADSL ORF mammalian expression plasmid, C-HA tag||HG11287-CY|
|Human ADSL Gene cDNA clone plasmid||HG11287-M|
|Human ADSL ORF mammalian expression plasmid, N-Flag tag||HG11287-NF|
|Human ADSL ORF mammalian expression plasmid, N-His tag||HG11287-NH|
|Human ADSL ORF mammalian expression plasmid, N-Myc tag||HG11287-NM|
|Human ADSL ORF mammalian expression plasmid, N-HA tag||HG11287-NY|
|Human ADSL natural ORF mammalian expression plasmid||HG11287-UT|
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Adenylosuccinate lyase, also known as adenylosuccinase, ADSL or ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the _-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by different mutations to the enzyme.