|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CRP ORF mammalian expression plasmid, C-GFPSpark tag||HG11250-ACG|
|Human CRP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11250-ACR|
|Human CRP ORF mammalian expression plasmid, C-Flag tag||HG11250-CF|
|Human CRP ORF mammalian expression plasmid, C-His tag||HG11250-CH|
|Human CRP ORF mammalian expression plasmid, C-Myc tag||HG11250-CM|
|Human CRP ORF mammalian expression plasmid, C-HA tag||HG11250-CY|
|Human CRP natural ORF mammalian expression plasmid||HG11250-M-N|
|Human CRP ORF mammalian expression plasmid, N-Flag tag||HG11250-NF|
|Human CRP ORF mammalian expression plasmid, N-His tag||HG11250-NH|
|Human CRP ORF mammalian expression plasmid, N-Myc tag||HG11250-NM|
|Human CRP ORF mammalian expression plasmid, N-HA tag||HG11250-NY|
|Human CRP natural ORF mammalian expression plasmid||HG11250-UT|
|Learn more about expression Vectors|
C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.