|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Cynomolgus monkey OPALIN ORF mammalian expression plasmid, C-GFPSpark tag||CG90667-ACG|
|Cynomolgus monkey OPALIN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90667-ACR|
|Cynomolgus monkey OPALIN ORF mammalian expression plasmid, C-Flag tag||CG90667-CF|
|Cynomolgus monkey OPALIN ORF mammalian expression plasmid, C-His tag||CG90667-CH|
|Cynomolgus monkey OPALIN ORF mammalian expression plasmid, C-Myc tag||CG90667-CM|
|Cynomolgus monkey OPALIN ORF mammalian expression plasmid, C-HA tag||CG90667-CY|
|Cynomolgus monkey OPALIN Gene cDNA clone plasmid||CG90667-G|
|Cynomolgus monkey OPALIN ORF mammalian expression plasmid, N-Flag tag||CG90667-NF|
|Cynomolgus monkey OPALIN ORF mammalian expression plasmid, N-His tag||CG90667-NH|
|Cynomolgus monkey OPALIN ORF mammalian expression plasmid, N-Myc tag||CG90667-NM|
|Cynomolgus monkey OPALIN ORF mammalian expression plasmid, N-HA tag||CG90667-NY|
|Cynomolgus monkey OPALIN natural ORF mammalian expression plasmid||CG90667-UT|
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Opalin, or oligodendrocytic myelin paranodal and inner loop protein, is a transmembrane protein detected specifically in mammalian oligodendrocytes, and may play significant role in oligodendrocyte differentiation and myelination.Opalin has binding sites for Myt1 and cAMP-response element binding protein (CREB). Over-expression of Myt1, treatment of the cell with leukemia inhibitory factor (LIF), and cAMP analog (CREB activator) enhanced the expression of endogenous Opalin in Oli-neu cells and activated the oligodendrocyte enhancer. Thus LIF, cAMP signaling cascades and Myt1 may play significant roles in the differentiation of oligodendrocytes through their action on the Opalin oligodendrocyte enhancer. Enzymatic deglycosylation showed that myelin Opalin contained N- and O-glycans, and that the O-glycans, at least, had negatively charged sialic acids. Site-directed mutations at the glycan sites impaired the cell surface localization of Opalin. In addition to the somata and processes of oligodendrocytes, Opalin immunoreactivity was observed in myelinated axons in a spiral fashion, and was concentrated in the paranodal loop region. Immunogold electron microscopy demonstrated that Opalin was localized at particular sites in the paranodal loop membrane. These results suggest a role for highly sialylglycosylated Opalin in an intermembranous function of the myelin paranodal loops in the central nervous system.