|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FOLR2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11219-ACG|
|Human FOLR2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11219-ACR|
|Human FOLR2 ORF mammalian expression plasmid, C-Flag tag||HG11219-CF|
|Human FOLR2 ORF mammalian expression plasmid, C-His tag||HG11219-CH|
|Human FOLR2 ORF mammalian expression plasmid, C-Myc tag||HG11219-CM|
|Human FOLR2 ORF mammalian expression plasmid, C-HA tag||HG11219-CY|
|Human FOLR2 Gene cDNA clone plasmid||HG11219-M|
|Human FOLR2 ORF mammalian expression plasmid, Flag tag||HG11219-M-F|
|Human FOLR2 ORF mammalian expression plasmid, N-Flag tag||HG11219-NF|
|Human FOLR2 ORF mammalian expression plasmid, N-His tag||HG11219-NH|
|Human FOLR2 ORF mammalian expression plasmid, N-Myc tag||HG11219-NM|
|Human FOLR2 ORF mammalian expression plasmid, N-HA tag||HG11219-NY|
|Human FOLR2 natural ORF mammalian expression plasmid||HG11219-UT|
|Learn more about expression Vectors|
Folate receptor beta, also known as Folate receptor 2, FBP, and FOLR2, is a member of the folate receptor family. FOLR2 is expressed in placenta and hematopoietic cells. The expression of FOLR2 is increased in malignant tissues. Members of the Folate receptor family members (FOLRs) have a high affinity for folic acid and for several reduced folic acid derivatives. They mediate the delivery of 5-methyltetrahydrofolate to the interior of, out of within, or between cells in a process known as potocytosis. FOLR2 has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. The FOLR2 protein was originally thought to exist only in placenta, but is also detected in spleen, bone marrow, and thymus. FOLR2 is a marker for macrophages generated in the presence of M-CSF, but not GM-CSF. Its expression correlates with increased folate uptake ability. Folate conjugates of therapeutic drugs are a potential immunotherapy tool to target tumor-associated macrophages.