|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PRMT3 ORF mammalian expression plasmid, C-GFPSpark tag||HG11211-ACG|
|Human PRMT3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11211-ACR|
|Human PRMT3 ORF mammalian expression plasmid, N-GFPSpark tag||HG11211-ANG|
|Human PRMT3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11211-ANR|
|Human PRMT3 ORF mammalian expression plasmid, C-Flag tag||HG11211-CF|
|Human PRMT3 ORF mammalian expression plasmid, C-His tag||HG11211-CH|
|Human PRMT3 ORF mammalian expression plasmid, C-Myc tag||HG11211-CM|
|Human PRMT3 ORF mammalian expression plasmid, C-HA tag||HG11211-CY|
|Human PRMT3 Gene cDNA clone plasmid||HG11211-M|
|Human PRMT3 ORF mammalian expression plasmid, Flag tag||HG11211-M-F|
|Human PRMT3 natural ORF mammalian expression plasmid||HG11211-M-N|
|Human PRMT3 ORF mammalian expression plasmid, N-Flag tag||HG11211-NF|
|Human PRMT3 ORF mammalian expression plasmid, N-His tag||HG11211-NH|
|Human PRMT3 ORF mammalian expression plasmid, N-Myc tag||HG11211-NM|
|Human PRMT3 ORF mammalian expression plasmid, N-HA tag||HG11211-NY|
|Human PRMT3 natural ORF mammalian expression plasmid||HG11211-UT|
|Learn more about expression Vectors|
Protein arginine methyltransferase 3, also known as PRMT3, is one of four type I protein arginine methyltransferases (PRMT) that in humans is encoded by the PRMT3 gene. Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation, signalling and subcellular localization of proteins and nucleic acids. In human cells, the PRMT family consists of eight canonical members. PRMTs have been classified into two groups based on the end product. Certain PRMTs display different subcellular localization in different cell types, implicating cell- and tissue-specific mechanisms for regulating PRMT functions. PRMT3 is unique in that its N-terminus harbours a C2H2 zinc-finger domain that is proposed to confer substrate specificity. In addition, PRMT3 is the only type I enzyme that is restricted to the cytoplasm. A large proportion of this cystosolic PRMT3 is found associated with ribosomes. It is tethered to the ribosomes through its interaction with rpS2, which is also its substrate.