|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Cynomolgus monkey BSG ORF mammalian expression plasmid, C-GFPSpark tag||CG90636-ACG|
|Cynomolgus monkey BSG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90636-ACR|
|Cynomolgus monkey BSG ORF mammalian expression plasmid, C-Flag tag||CG90636-CF|
|Cynomolgus monkey BSG ORF mammalian expression plasmid, C-His tag||CG90636-CH|
|Cynomolgus monkey BSG ORF mammalian expression plasmid, C-Myc tag||CG90636-CM|
|Cynomolgus monkey BSG ORF mammalian expression plasmid, C-HA tag||CG90636-CY|
|Cynomolgus monkey BSG Gene cDNA clone plasmid||CG90636-G|
|Cynomolgus monkey BSG ORF mammalian expression plasmid, N-Flag tag||CG90636-NF|
|Cynomolgus monkey BSG ORF mammalian expression plasmid, N-His tag||CG90636-NH|
|Cynomolgus monkey BSG ORF mammalian expression plasmid, N-Myc tag||CG90636-NM|
|Cynomolgus monkey BSG ORF mammalian expression plasmid, N-HA tag||CG90636-NY|
|Cynomolgus monkey BSG natural ORF mammalian expression plasmid||CG90636-UT|
|Learn more about expression Vectors|
CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.