|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human NEGR1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11181-ACG|
|Human NEGR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11181-ACR|
|Human NEGR1 ORF mammalian expression plasmid, C-Flag tag||HG11181-CF|
|Human NEGR1 ORF mammalian expression plasmid, C-His tag||HG11181-CH|
|Human NEGR1 ORF mammalian expression plasmid, C-Myc tag||HG11181-CM|
|Human NEGR1 ORF mammalian expression plasmid, C-HA tag||HG11181-CY|
|Human NEGR1 Gene cDNA clone plasmid||HG11181-M|
|Human NEGR1 ORF mammalian expression plasmid, N-Flag tag||HG11181-NF|
|Human NEGR1 ORF mammalian expression plasmid, N-His tag||HG11181-NH|
|Human NEGR1 ORF mammalian expression plasmid, N-Myc tag||HG11181-NM|
|Human NEGR1 ORF mammalian expression plasmid, N-HA tag||HG11181-NY|
|Human NEGR1 natural ORF mammalian expression plasmid||HG11181-UT|
|Learn more about expression Vectors|
Neuronal Growth Regulator 1, NEGR1, also known as neurotractin, or KILON, which belongs to the immunoglobulin superfamily, IgLON family. This GPI-linked cell surface glycoprotein NEGR1 is composed of three Ig-like domains and belongs to the IgLON subgroup of neural IgSF members. It is expressed in two isoforms with apparent molecular masses of 50 and 37 kD, termed L-form and S-form, respectively. NEGR1/Neurotractin participates in the regulation of neurite outgrowth in the developing brain, and is expressed on neurites of primary hippocampal neurons. Neurotractin/KILON is a trans-neural growth-promoting factor for outgrowing axons following hippocampal denervation. KILON (kindred of IgLON) and opioid-binding cell adhesion molecule belong to the IgLON subgroup of immunoglobulin superfamily together with the limbic system-associated membrane protein and neurotrimin. The alteration of modulatory function of KILON/NEGR1 for the number of dendritic synapses concomitant with changes in its localization and detergent solubility during neuronal culture development. In addition to its reported role in the brain, NEGR1 is also expressed in subcutaneous adipose tissue and acts as a central 'hub' in an obesity-related transcript network.