|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GADD45A ORF mammalian expression plasmid, C-GFPSpark tag||HG11156-ACG|
|Human GADD45A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11156-ACR|
|Human GADD45A ORF mammalian expression plasmid, N-GFPSpark tag||HG11156-ANG|
|Human GADD45A ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11156-ANR|
|Human GADD45A ORF mammalian expression plasmid, C-Flag tag||HG11156-CF|
|Human GADD45A ORF mammalian expression plasmid, C-His tag||HG11156-CH|
|Human GADD45A ORF mammalian expression plasmid, C-Myc tag||HG11156-CM|
|Human GADD45A ORF mammalian expression plasmid, C-HA tag||HG11156-CY|
|Human GADD45A Gene cDNA clone plasmid||HG11156-M|
|Human GADD45A ORF mammalian expression plasmid, N-Flag tag||HG11156-NF|
|Human GADD45A ORF mammalian expression plasmid, N-His tag||HG11156-NH|
|Human GADD45A ORF mammalian expression plasmid, N-Myc tag||HG11156-NM|
|Human GADD45A ORF mammalian expression plasmid, N-HA tag||HG11156-NY|
|Human GADD45A natural ORF mammalian expression plasmid||HG11156-UT|
|Learn more about expression Vectors|
GADD45A is a member of the GADD45 Family, and has been found to associate with several cytoplasmic and nuclear factors and has been implicated in several cellular functions, including MAPK signaling, cell cycle regulation, DNA repair and genomic stability, apoptosis, and immune responses. The GADD45 Family of genes is rapidly induced by different stressors, including differentiation-inducing cytokines, and there is a large body of evidence that their cognate proteins are key players in cellular stress responses. GADD45A protein has been reported to interact with multiple important cellular proteins, including Cdc2 protein kinase, proliferating cell nuclear antigen (PCNA), p21Waf1/Cip1 protein, core histone protein and MTK/MEKK4, an up-stream activator of the JNK/SAPK pathway, indicating that GADD45A may play important roles in the control of cell cycle checkpoint, DNA repair process, and signaling transduction. GADD45A expression in response to genotoxic stress illustrates a more complex scenario, wherein transcriptional changes operate in concert with mRNA turnover and translational regulation. GADD45A was the first stress-inducible gene determined to be up-regulated by p53 and is also a target for the p53 homologues, p63 and p73. The decreased GADD45A expression is also considered a survival mechanism, as cancer cells without this control can evade the apoptotic pathway leading to increased tumourigenesis. As GADD45A is an essential component of many metabolic pathways that control proliferating cancer cells, it presents itself as an emerging drug target worthy of further investigation.