|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human S100A12 ORF mammalian expression plasmid, C-GFPSpark tag||HG11143-ACG|
|Human S100A12 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11143-ACR|
|Human S100A12 ORF mammalian expression plasmid, C-Flag tag||HG11143-CF|
|Human S100A12 ORF mammalian expression plasmid, C-His tag||HG11143-CH|
|Human S100A12 ORF mammalian expression plasmid, C-Myc tag||HG11143-CM|
|Human S100A12 ORF mammalian expression plasmid, C-HA tag||HG11143-CY|
|Human S100A12 Gene cDNA clone plasmid||HG11143-M|
|Human S100A12 ORF mammalian expression plasmid, Flag tag||HG11143-M-F|
|Human S100A12 ORF mammalian expression plasmid, N-Flag tag||HG11143-NF|
|Human S100A12 ORF mammalian expression plasmid, N-His tag||HG11143-NH|
|Human S100A12 ORF mammalian expression plasmid, N-Myc tag||HG11143-NM|
|Human S100A12 ORF mammalian expression plasmid, N-HA tag||HG11143-NY|
|Human S100A12 natural ORF mammalian expression plasmid||HG11143-UT|
|Learn more about expression Vectors|
S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.
Protein S100-A12, also known as S100 calcium-binding protein A12, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, and S100A12, is a member of the S-101 family. Like the majority of S100 proteins, S100A12 is a dimer, with the interface between the two subunits being composed mostly of hydrophobic residues. The fold of S100A12 is similar to the other known crystal and solution structures of S100 proteins, except for the linker region between the two EF-hand motifs. S100A12 plays an important role in the inflammatory response.