|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SERPINI1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11107-ACG|
|Human SERPINI1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11107-ACR|
|Human SERPINI1 ORF mammalian expression plasmid, C-Flag tag||HG11107-CF|
|Human SERPINI1 ORF mammalian expression plasmid, C-His tag||HG11107-CH|
|Human SERPINI1 ORF mammalian expression plasmid, C-Myc tag||HG11107-CM|
|Human SERPINI1 ORF mammalian expression plasmid, C-HA tag||HG11107-CY|
|Human SERPINI1 Gene cDNA clone plasmid||HG11107-M|
|Human SERPINI1 ORF mammalian expression plasmid, N-Flag tag||HG11107-NF|
|Human SERPINI1 ORF mammalian expression plasmid, N-His tag||HG11107-NH|
|Human SERPINI1 ORF mammalian expression plasmid, N-Myc tag||HG11107-NM|
|Human SERPINI1 ORF mammalian expression plasmid, N-HA tag||HG11107-NY|
|Human SERPINI1 natural ORF mammalian expression plasmid||HG11107-UT|
|Learn more about expression Vectors|
Neuroserpin, also known as Protease inhibitor 12 and SERPINI1, is a secreted protein which belongs to the serpin family. Neuroserpin is a serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. Serine protease inhibitors of the serpin superfamily are involved in many cellular processes. Neuroserpin was first identified as a protein secreted from the axons of dorsal root ganglion neurons. Neuroserpin is predominantly expressed in the brain, and is expressed in the late stages of neurogenesis during the process of synapse formation. Overexpression of neuroserpin in an anterior pituitary corticotroph cell line results in the extension of neurite-like processes, suggesting that neuroserpin may play a role in cell communication, cell adhesion, and/or cell migration. Neuroserpin may be involved in the formation or reorganization of synaptic connections, as well as synaptic plasticity in the adult nervous system. Neuroserpin may also protect neurons from cell damage by tissue-type plasminogen activator. Defects of neuroserpin are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B).