|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TGM2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11095-ACG|
|Human TGM2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11095-ACR|
|Human TGM2 ORF mammalian expression plasmid, N-GFPSpark tag||HG11095-ANG|
|Human TGM2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11095-ANR|
|Human TGM2 ORF mammalian expression plasmid, C-Flag tag||HG11095-CF|
|Human TGM2 ORF mammalian expression plasmid, C-His tag||HG11095-CH|
|Human TGM2 ORF mammalian expression plasmid, C-Myc tag||HG11095-CM|
|Human TGM2 ORF mammalian expression plasmid, C-HA tag||HG11095-CY|
|Human TGM2 Gene cDNA clone plasmid||HG11095-M|
|Human TGM2 ORF mammalian expression plasmid, Flag tag||HG11095-M-F|
|Human TGM2 ORF mammalian expression plasmid, N-Flag tag||HG11095-NF|
|Human TGM2 ORF mammalian expression plasmid, N-His tag||HG11095-NH|
|Human TGM2 ORF mammalian expression plasmid, N-Myc tag||HG11095-NM|
|Human TGM2 ORF mammalian expression plasmid, N-HA tag||HG11095-NY|
|Human TGM2 natural ORF mammalian expression plasmid||HG11095-UT|
|Learn more about expression Vectors|
Protein-glutamine gamma-glutamyltransferase 2, also known as Tissue transglutaminase, Transglutaminase C, Transglutaminase-2, and TGM2, is a member of the transglutaminase superfamily. TGM2 plays a role in cell growth and survival through the anti-apoptosis signaling pathway. It is a calcium-dependent acyltransferase which also undergoes a GTP-binding/GTPase cycle even though it lacks any obvious sequence similarity with canonical GTP-binding (G) proteins. TGM2 is a multi-functional protein which catalyzes transamidation reactions or acts as a G-protein in intracellular signalling. As an enzyme which is responsible for the majority of transglutaminase (TG) activity in the brain, TGM2 is likely to play a modulatory role in nervous system development and has regulatory effect on neuronal cell death as well. Most importantly, numerous studies have presented data demonstrating that dysregulation of TGM2 may contribute to the pathogenesis of many neurodegenerative disorders, including Huntington's disease, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis as well as nervous system injuries.