|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus CPB1 ORF mammalian expression plasmid, C-GFPSpark tag||CG90524-ACG|
|Rhesus CPB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90524-ACR|
|Rhesus CPB1 ORF mammalian expression plasmid, N-GFPSpark tag||CG90524-ANG|
|Rhesus CPB1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||CG90524-ANR|
|Rhesus CPB1 ORF mammalian expression plasmid, C-Flag tag||CG90524-CF|
|Rhesus CPB1 ORF mammalian expression plasmid, C-His tag||CG90524-CH|
|Rhesus CPB1 ORF mammalian expression plasmid, C-Myc tag||CG90524-CM|
|Rhesus CPB1 ORF mammalian expression plasmid, C-HA tag||CG90524-CY|
|Rhesus CPB1 ORF mammalian expression plasmid, N-Flag tag||CG90524-NF|
|Rhesus CPB1 ORF mammalian expression plasmid, N-His tag||CG90524-NH|
|Rhesus CPB1 ORF mammalian expression plasmid, N-Myc tag||CG90524-NM|
|Rhesus CPB1 ORF mammalian expression plasmid, N-HA tag||CG90524-NY|
|Rhesus CPB1 Gene cDNA clone plasmid||CG90524-U|
|Rhesus CPB1 natural ORF mammalian expression plasmid||CG90524-UT|
|Learn more about expression Vectors|
Human Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.