|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PADI4 ORF mammalian expression plasmid, C-GFPSpark tag||HG11072-ACG|
|Human PADI4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11072-ACR|
|Human PADI4 ORF mammalian expression plasmid, N-GFPSpark tag||HG11072-ANG|
|Human PADI4 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11072-ANR|
|Human PADI4 ORF mammalian expression plasmid, C-Flag tag||HG11072-CF|
|Human PADI4 ORF mammalian expression plasmid, C-His tag||HG11072-CH|
|Human PADI4 ORF mammalian expression plasmid, C-Myc tag||HG11072-CM|
|Human PADI4 ORF mammalian expression plasmid, C-HA tag||HG11072-CY|
|Human PADI4 Gene cDNA clone plasmid||HG11072-M|
|Human PADI4 ORF mammalian expression plasmid, Myc tag||HG11072-M-M|
|Human PADI4 ORF mammalian expression plasmid, N-Flag tag||HG11072-NF|
|Human PADI4 ORF mammalian expression plasmid, N-His tag||HG11072-NH|
|Human PADI4 ORF mammalian expression plasmid, N-Myc tag||HG11072-NM|
|Human PADI4 ORF mammalian expression plasmid, N-HA tag||HG11072-NY|
|Human PADI4 natural ORF mammalian expression plasmid||HG11072-UT|
|Learn more about expression Vectors|
Protein-arginine deiminase type-4, also known as HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type I V and PADI4, is a cytoplasm and nucleus protein which belongs to the protein arginine deiminase family. PADI4 is expressed in CD34+ stem cells in normal tissues, and many more CD34+ cells expressing PADI4 are present in tumour tissues. PADI4 post-translationally converts peptidylarginine to citrulline, a process called citrullination. Studies have demonstrated the high expression of PADI4 in various malignant tumour tissues. PADI4 is also expressed at high levels in the blood of patients with some malignant tumours. Citrullination of histone, cytokeratin, antithrombin and fibronectin have been confirmed to be involved in abnormal apoptosis, high coagulation, and disordered cell proliferation and differentiation, all of which are main features of malignant tumours. PADI4 may play an important role in tumourigenesis. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.