|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GFPT1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11071-ACG|
|Human GFPT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11071-ACR|
|Human GFPT1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11071-ANG|
|Human GFPT1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11071-ANR|
|Human GFPT1 ORF mammalian expression plasmid, C-Flag tag||HG11071-CF|
|Human GFPT1 ORF mammalian expression plasmid, C-His tag||HG11071-CH|
|Human GFPT1 ORF mammalian expression plasmid, C-Myc tag||HG11071-CM|
|Human GFPT1 ORF mammalian expression plasmid, C-HA tag||HG11071-CY|
|Human GFPT1 Gene cDNA clone plasmid||HG11071-M|
|Human GFPT1 ORF mammalian expression plasmid, N-Flag tag||HG11071-NF|
|Human GFPT1 ORF mammalian expression plasmid, N-His tag||HG11071-NH|
|Human GFPT1 ORF mammalian expression plasmid, N-Myc tag||HG11071-NM|
|Human GFPT1 ORF mammalian expression plasmid, N-HA tag||HG11071-NY|
|Human GFPT1 natural ORF mammalian expression plasmid||HG11071-UT|
|Learn more about expression Vectors|
Glutamine:fructose-6-phosphate amidotransferase 1 (GFAT), also known as GFPT1, is a member of the N-terminal nucleophile aminotransferases and the first rate-limiting enzyme for the entry of glucose into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT transfers the amino group from the L-glutamine amide to the D-fructose 6-phosphate, producing glutamic acid and glucosamine 6-phosphate. GFAT exists as a homotetramer in cytoplasm, and is proposed to be most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. The full length of human GFAT contains 1 glutamine amidotransferase type-2 domain which catalyzes amide nitrogen transfer from glutamine to the appropriate substrate, and 2 SIS (Sugar Isomerase) domains found in many phosphosugar isomerases and phosphosugar binding proteins.Two isoforms of gfat have been identified: GFAT1 is predominantly expressed in skeletal muscle, whereas GFAT2 is expressed mainly in the central nervous system.