|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SCARB1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11069-ACG|
|Human SCARB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11069-ACR|
|Human SCARB1 ORF mammalian expression plasmid, C-Flag tag||HG11069-CF|
|Human SCARB1 ORF mammalian expression plasmid, C-His tag||HG11069-CH|
|Human SCARB1 ORF mammalian expression plasmid, C-Myc tag||HG11069-CM|
|Human SCARB1 ORF mammalian expression plasmid, C-HA tag||HG11069-CY|
|Human SCARB1 Gene cDNA Clone (full-length ORF Clone)||HG11069-M|
|Human SCARB1 ORF mammalian expression plasmid, Flag tag||HG11069-M-F|
|Human SCARB1 natural ORF mammalian expression plasmid||HG11069-M-N|
|Human SCARB1 ORF mammalian expression plasmid, N-Flag tag||HG11069-NF|
|Human SCARB1 ORF mammalian expression plasmid, N-His tag||HG11069-NH|
|Human SCARB1 ORF mammalian expression plasmid, N-Myc tag||HG11069-NM|
|Human SCARB1 ORF mammalian expression plasmid, N-HA tag||HG11069-NY|
|Human SCARB1 natural ORF mammalian expression plasmid||HG11069-UT|
|Learn more about expression Vectors|
Scavenger receptor class B, member 1 (SCARB1), also known as CD36L1, is a member of the scavenger receptor family. SCARB1 is expressed primarily in liver and non placental steroidogenic tissues, and predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane. SCARB1 is proposed as a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells, and is involved in a wide variety of physilogical processes. As a key component in the reverse cholesterol transport pathway, SCARB1 binds high density lipoproteins (HDLs) and mediates selective cholesterol uptake by a mechanism distinct from the LDL pathway. High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SCARB1 may thus serve as a useful marker that predicts variation in baseline lipid levels and postprandial lipid response. The mouse SCARB1 has been shown to exert actions in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.