|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IL32 ORF mammalian expression plasmid, C-GFPSpark tag||HG10860-ACG|
|Human IL32 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10860-ACR|
|Human IL32 ORF mammalian expression plasmid, C-Flag tag||HG10860-CF|
|Human IL32 ORF mammalian expression plasmid, C-His tag||HG10860-CH|
|Human IL32 ORF mammalian expression plasmid, C-Myc tag||HG10860-CM|
|Human IL32 ORF mammalian expression plasmid, C-HA tag||HG10860-CY|
|Human IL32 Gene cDNA clone plasmid||HG10860-G|
|Human IL32 ORF mammalian expression plasmid, N-Flag tag||HG10860-NF|
|Human IL32 ORF mammalian expression plasmid, N-His tag||HG10860-NH|
|Human IL32 ORF mammalian expression plasmid, N-Myc tag||HG10860-NM|
|Human IL32 ORF mammalian expression plasmid, N-HA tag||HG10860-NY|
|Human IL32 natural ORF mammalian expression plasmid||HG10860-UT|
|Learn more about expression Vectors|
IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines, and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma and delta) are the most representative IL-32 transcripts, and gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis and in human stomach cancer, human lung cancer and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.