|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus ADAM8 ORF mammalian expression plasmid, C-GFPSpark tag||CG90278-ACG|
|Rhesus ADAM8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90278-ACR|
|Rhesus ADAM8 ORF mammalian expression plasmid, C-Flag tag||CG90278-CF|
|Rhesus ADAM8 ORF mammalian expression plasmid, C-His tag||CG90278-CH|
|Rhesus ADAM8 ORF mammalian expression plasmid, C-Myc tag||CG90278-CM|
|Rhesus ADAM8 ORF mammalian expression plasmid, C-HA tag||CG90278-CY|
|Rhesus ADAM8 Gene cDNA clone plasmid||CG90278-G|
|Rhesus ADAM8 ORF mammalian expression plasmid, N-Flag tag||CG90278-NF|
|Rhesus ADAM8 ORF mammalian expression plasmid, N-His tag||CG90278-NH|
|Rhesus ADAM8 ORF mammalian expression plasmid, N-Myc tag||CG90278-NM|
|Rhesus ADAM8 ORF mammalian expression plasmid, N-HA tag||CG90278-NY|
|Rhesus ADAM8 natural ORF mammalian expression plasmid||CG90278-UT|
|Learn more about expression Vectors|
ADAM8, also known as CD156, is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM8 is possiblely involved in extravasation of leukocytes As a metalloprotease, ADAM8 also may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma.