|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FGFR2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10824-ACG|
|Human FGFR2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10824-ACR|
|Human FGFR2 ORF mammalian expression plasmid, C-Flag tag||HG10824-CF|
|Human FGFR2 ORF mammalian expression plasmid, C-His tag||HG10824-CH|
|Human FGFR2 ORF mammalian expression plasmid, C-Myc tag||HG10824-CM|
|Human FGFR2 ORF mammalian expression plasmid, C-HA tag||HG10824-CY|
|Human FGFR2 Gene cDNA clone plasmid||HG10824-M|
|Human FGFR2 ORF mammalian expression plasmid, N-Flag tag||HG10824-NF|
|Human FGFR2 ORF mammalian expression plasmid, N-His tag||HG10824-NH|
|Human FGFR2 ORF mammalian expression plasmid, N-Myc tag||HG10824-NM|
|Human FGFR2 ORF mammalian expression plasmid, N-HA tag||HG10824-NY|
|Human FGFR2 natural ORF mammalian expression plasmid||HG10824-UT|
|Learn more about expression Vectors|
FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.