|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CXADR ORF mammalian expression plasmid, C-GFPSpark tag||HG10799-ACG|
|Human CXADR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10799-ACR|
|Human CXADR ORF mammalian expression plasmid, C-Flag tag||HG10799-CF|
|Human CXADR ORF mammalian expression plasmid, C-His tag||HG10799-CH|
|Human CXADR ORF mammalian expression plasmid, C-Myc tag||HG10799-CM|
|Human CXADR ORF mammalian expression plasmid, C-HA tag||HG10799-CY|
|Human CXADR Gene cDNA clone plasmid||HG10799-M|
|Human CXADR ORF mammalian expression plasmid, N-Flag tag||HG10799-NF|
|Human CXADR ORF mammalian expression plasmid, N-His tag||HG10799-NH|
|Human CXADR ORF mammalian expression plasmid, N-Myc tag||HG10799-NM|
|Human CXADR ORF mammalian expression plasmid, N-HA tag||HG10799-NY|
|Human CXADR natural ORF mammalian expression plasmid||HG10799-UT|
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CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 90% sequence identity with the corresponding regions of rat, human CXADR.