|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus IL6 ORF mammalian expression plasmid, C-GFPSpark tag||CG90197-ACG|
|Rhesus IL6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90197-ACR|
|Rhesus IL6 ORF mammalian expression plasmid, C-Flag tag||CG90197-CF|
|Rhesus IL6 ORF mammalian expression plasmid, C-His tag||CG90197-CH|
|Rhesus IL6 ORF mammalian expression plasmid, C-Myc tag||CG90197-CM|
|Rhesus IL6 ORF mammalian expression plasmid, C-HA tag||CG90197-CY|
|Rhesus IL6 Gene cDNA clone plasmid||CG90197-G|
|Rhesus IL6 ORF mammalian expression plasmid, N-Flag tag||CG90197-NF|
|Rhesus IL6 ORF mammalian expression plasmid, N-His tag||CG90197-NH|
|Rhesus IL6 ORF mammalian expression plasmid, N-Myc tag||CG90197-NM|
|Rhesus IL6 ORF mammalian expression plasmid, N-HA tag||CG90197-NY|
|Rhesus IL6 natural ORF mammalian expression plasmid||CG90197-UT|
|Learn more about expression Vectors|
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.