|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human sFRP-2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10756-ACG|
|Human sFRP-2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10756-ACR|
|Human sFRP-2 ORF mammalian expression plasmid, C-Flag tag||HG10756-CF|
|Human sFRP-2 ORF mammalian expression plasmid, C-His tag||HG10756-CH|
|Human sFRP-2 ORF mammalian expression plasmid, C-Myc tag||HG10756-CM|
|Human sFRP-2 ORF mammalian expression plasmid, C-HA tag||HG10756-CY|
|Human sFRP-2 Gene cDNA clone plasmid||HG10756-M|
|Human sFRP-2 ORF mammalian expression plasmid, N-Flag tag||HG10756-NF|
|Human sFRP-2 ORF mammalian expression plasmid, N-His tag||HG10756-NH|
|Human sFRP-2 ORF mammalian expression plasmid, N-Myc tag||HG10756-NM|
|Human sFRP-2 ORF mammalian expression plasmid, N-HA tag||HG10756-NY|
|Human sFRP-2 natural ORF mammalian expression plasmid||HG10756-UT|
|Learn more about expression Vectors|
The Secreted frizzled-related protein (SFRP) family consists of five secreted glycoproteins in humans (SFRP1~5) that act as extracellular signaling ligands. Each SFRP is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. sFRP2 is a member of the SFRP family acting as soluble modulators of Wnt signaling and contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain.sFRP2 inhibites hypoxia induced endothelial cell apoptosis and increases endothelial cell migration. It prevents mesoderm specification and maintains the cells in the undifferentiated state. SFRP2 is also a novel stimulator of angiogenesis that stimulates angiogenesis via a calcineurin/NFAT pathway, thus is regarded as a favorable target for the inhibition of angiogenesis in solid tumors. Mouse sFRP2 is highly expressed in the eye and is also detected in heart and lung at low level.