|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human VTCN1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10738-ACG|
|Human VTCN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10738-ACR|
|Human VTCN1 ORF mammalian expression plasmid, C-Flag tag||HG10738-CF|
|Human VTCN1 ORF mammalian expression plasmid, C-His tag||HG10738-CH|
|Human VTCN1 ORF mammalian expression plasmid, C-Myc tag||HG10738-CM|
|Human VTCN1 ORF mammalian expression plasmid, C-HA tag||HG10738-CY|
|Human VTCN1 Gene cDNA clone plasmid||HG10738-M|
|Human VTCN1 natural ORF mammalian expression plasmid||HG10738-M-N|
|Human VTCN1 ORF mammalian expression plasmid, N-Flag tag||HG10738-NF|
|Human VTCN1 ORF mammalian expression plasmid, N-His tag||HG10738-NH|
|Human VTCN1 ORF mammalian expression plasmid, N-Myc tag||HG10738-NM|
|Human VTCN1 ORF mammalian expression plasmid, N-HA tag||HG10738-NY|
|Human VTCN1 natural ORF mammalian expression plasmid||HG10738-UT|
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V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type? membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X / VTCN1 / B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X / VTCN1 / B7H4 plays an important role, together with regulatory T-cells(Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X / VTCN1 / B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6 / interleukin-6 and IL10 / interleukin-10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells.