|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TRIB3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10731-ACG|
|Human TRIB3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10731-ACR|
|Human TRIB3 ORF mammalian expression plasmid, N-GFPSpark tag||HG10731-ANG|
|Human TRIB3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10731-ANR|
|Human TRIB3 ORF mammalian expression plasmid, C-Flag tag||HG10731-CF|
|Human TRIB3 ORF mammalian expression plasmid, C-His tag||HG10731-CH|
|Human TRIB3 ORF mammalian expression plasmid, C-Myc tag||HG10731-CM|
|Human TRIB3 ORF mammalian expression plasmid, C-HA tag||HG10731-CY|
|Human TRIB3 ORF mammalian expression plasmid, Flag tag||HG10731-M-F|
|Human TRIB3 natural ORF mammalian expression plasmid||HG10731-M-N|
|Human TRIB3 ORF mammalian expression plasmid, N-Flag tag||HG10731-NF|
|Human TRIB3 ORF mammalian expression plasmid, N-His tag||HG10731-NH|
|Human TRIB3 ORF mammalian expression plasmid, N-Myc tag||HG10731-NM|
|Human TRIB3 ORF mammalian expression plasmid, N-HA tag||HG10731-NY|
|Human TRIB3 natural ORF mammalian expression plasmid||HG10731-UT|
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Tribbles homolog 3, also known as Neuronal cell death-inducible putative kinase, p65-interacting inhibitor of NF-kappa-B, SINK and TRIB3, is a Nucleus protein which belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family and Tribbles subfamily. Highest expression Of TRIB3 is in liver, pancreas, peripheral blood leukocytes and bone marrow. It is also highly expressed in a number of primary lung, colon and breast tumors. TRIB3 is expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney. TRIB3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. TRIB3 may bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. It binds to ATF4 and inhibits its transcriptional activation activity. TRIB3 interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. It interacts with MAPK kinases and regulates activation of MAP kinases. It may play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. TRIB3 does not display kinase activity.