|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human DYRK3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10726-ACG|
|Human DYRK3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10726-ACR|
|Human DYRK3 ORF mammalian expression plasmid, N-GFPSpark tag||HG10726-ANG|
|Human DYRK3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10726-ANR|
|Human DYRK3 ORF mammalian expression plasmid, C-Flag tag||HG10726-CF|
|Human DYRK3 ORF mammalian expression plasmid, C-His tag||HG10726-CH|
|Human DYRK3 ORF mammalian expression plasmid, C-Myc tag||HG10726-CM|
|Human DYRK3 ORF mammalian expression plasmid, C-HA tag||HG10726-CY|
|Human DYRK3 Gene cDNA clone plasmid||HG10726-M|
|Human DYRK3 ORF mammalian expression plasmid, Flag tag||HG10726-M-F|
|Human DYRK3 ORF mammalian expression plasmid, N-Flag tag||HG10726-NF|
|Human DYRK3 ORF mammalian expression plasmid, N-His tag||HG10726-NH|
|Human DYRK3 ORF mammalian expression plasmid, N-Myc tag||HG10726-NM|
|Human DYRK3 ORF mammalian expression plasmid, N-HA tag||HG10726-NY|
|Human DYRK3 natural ORF mammalian expression plasmid||HG10726-UT|
|Learn more about expression Vectors|
Dual specificity tyrosine-phosphorylation-regulated kinase 3, also known as Regulatory erythroid kinase, REDK and DYRK3, is a nucleus protein which belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MNB/DYRK subfamily. DYRKs are an emerging family of dual-specificity kinases that play key roles in cell proliferation, survival, and development. DYRK3 contains one protein kinase domain. Isoform 1 and isoform 2 of DYRK3 are highly expressed in testis and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 of DYRK3 is the predominant form in testis. Isoform 1 of DYRK3 is the predominant form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low levels in heart, pancreas, lymph node, and thymus. DYRK3 is a negative regulator of EPO-dependent erythropoiesis. It may place an upper limit on red cell production during stress erythropoiesis. DYRK3 inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. It may also act by regulating CREB/CRE signaling. DYRK3 proved to effectively inhibit NFAT (nuclear factor of activated T cells) transcriptional response pathways and to co-immunoprecipitate with NFATc3. DYRK3 attenuates (and possibly apportions) red cell production selectively during anemia.