|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Cynomolgus monkey CDH17 ORF mammalian expression plasmid, C-GFPSpark tag||CG90147-ACG|
|Cynomolgus monkey CDH17 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90147-ACR|
|Cynomolgus monkey CDH17 ORF mammalian expression plasmid, C-Flag tag||CG90147-CF|
|Cynomolgus monkey CDH17 ORF mammalian expression plasmid, C-His tag||CG90147-CH|
|Cynomolgus monkey CDH17 ORF mammalian expression plasmid, C-Myc tag||CG90147-CM|
|Cynomolgus monkey CDH17 ORF mammalian expression plasmid, C-HA tag||CG90147-CY|
|Cynomolgus monkey CDH17 Gene cDNA clone plasmid||CG90147-G|
|Cynomolgus monkey CDH17 ORF mammalian expression plasmid, N-Flag tag||CG90147-NF|
|Cynomolgus monkey CDH17 ORF mammalian expression plasmid, N-His tag||CG90147-NH|
|Cynomolgus monkey CDH17 ORF mammalian expression plasmid, N-Myc tag||CG90147-NM|
|Cynomolgus monkey CDH17 ORF mammalian expression plasmid, N-HA tag||CG90147-NY|
|Cynomolgus monkey CDH17 natural ORF mammalian expression plasmid||CG90147-UT|
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Cadherin-17 or LI-cadherin is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Cadherin-17/LI-cadherin is a cadherin-like protein consisting of an extracellular region, 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing of the encoding gene results in multiple transcript variants. Cadherin-17/LI-cadherin preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin-17 may thus contribute to the sorting of heterogeneous cell types and have a role in the morphological organization of liver and intestine. It's also involved in intestinal peptide transport. Experiments have reported the association between Cadherin-17/LI-cadherin and gastric cancer. Cadherin-17/LI-cadherin expression was detected in 63/94 of gastric adenocarcinomas in addition to intestinal metaplasia. The expression of Cadherin-17 tended to be associated with intestinal type carcinoma, and carcinomas with Cadherin-17 expression was significantly more frequent in advanced stage cases than in early stage. Cadherin-17 is also a useful immunohistochemical marker for diagnosis of adenocarcinomas of the digestive system.