|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus IL18RAP ORF mammalian expression plasmid, C-GFPSpark tag||CG90122-ACG|
|Rhesus IL18RAP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90122-ACR|
|Rhesus IL18RAP ORF mammalian expression plasmid, C-Flag tag||CG90122-CF|
|Rhesus IL18RAP ORF mammalian expression plasmid, C-His tag||CG90122-CH|
|Rhesus IL18RAP ORF mammalian expression plasmid, C-Myc tag||CG90122-CM|
|Rhesus IL18RAP ORF mammalian expression plasmid, C-HA tag||CG90122-CY|
|Rhesus IL18RAP Gene cDNA clone plasmid||CG90122-G|
|Rhesus IL18RAP ORF mammalian expression plasmid, N-Flag tag||CG90122-NF|
|Rhesus IL18RAP ORF mammalian expression plasmid, N-His tag||CG90122-NH|
|Rhesus IL18RAP ORF mammalian expression plasmid, N-Myc tag||CG90122-NM|
|Rhesus IL18RAP ORF mammalian expression plasmid, N-HA tag||CG90122-NY|
|Rhesus IL18RAP natural ORF mammalian expression plasmid||CG90122-UT|
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Interleukin 18 receptor accessory protein, also known as IL18RAP and CDw218b (cluster of differentiation w218b), is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand binding subunit of IL18 receptor. The coexpression of IL18R1 and this protein is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18. IL18RAP is required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex. IL18RAP together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. Two putative isoforms of IL18RAP were detected and the ratios and total levels of these isoforms may contribute to the aetiology of coeliac disease. IL18R1 and IL18RAP polymorphisms have been found associated with diseases such as schizophrenia, HSV1 seropositivity and atopic asthma. Analysis of IL18R1 and IL18RAP SNPs in 5 European prospective cohorts suggests that the variability of these genes are unlikely to contribute to modulate the risk of CVD in European populations.