|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human Follistatin ORF mammalian expression plasmid, C-GFPSpark tag||HG10685-ACG|
|Human Follistatin ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10685-ACR|
|Human Follistatin ORF mammalian expression plasmid, C-Flag tag||HG10685-CF|
|Human Follistatin ORF mammalian expression plasmid, C-His tag||HG10685-CH|
|Human Follistatin ORF mammalian expression plasmid, C-Myc tag||HG10685-CM|
|Human Follistatin ORF mammalian expression plasmid, C-HA tag||HG10685-CY|
|Human Follistatin ORF mammalian expression plasmid, N-Flag tag||HG10685-NF|
|Human Follistatin ORF mammalian expression plasmid, N-His tag||HG10685-NH|
|Human Follistatin ORF mammalian expression plasmid, N-Myc tag||HG10685-NM|
|Human Follistatin ORF mammalian expression plasmid, N-HA tag||HG10685-NY|
|Human Follistatin natural ORF mammalian expression plasmid||HG10685-UT|
|Learn more about expression Vectors|
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. follistatin are expressed and subserve local regulatory roles in numerous extragonadal tissues, including brain, adrenal, bone marrow, and placenta but perhaps most notably in anterior pituitary-the classical target tissue for inhibin, the activin-follistatin system may play a key role in early embryogenesis. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone follistatin is a binding protein to activin. Since activin binds to follistatin, it is imperative to determine the nature of the activin/follistatin binding complex.