|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus IL10RB ORF mammalian expression plasmid, C-GFPSpark tag||CG90116-ACG|
|Rhesus IL10RB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90116-ACR|
|Rhesus IL10RB ORF mammalian expression plasmid, C-Flag tag||CG90116-CF|
|Rhesus IL10RB ORF mammalian expression plasmid, C-His tag||CG90116-CH|
|Rhesus IL10RB ORF mammalian expression plasmid, C-Myc tag||CG90116-CM|
|Rhesus IL10RB ORF mammalian expression plasmid, C-HA tag||CG90116-CY|
|Rhesus IL10RB Gene cDNA clone plasmid||CG90116-G|
|Rhesus IL10RB ORF mammalian expression plasmid, N-Flag tag||CG90116-NF|
|Rhesus IL10RB ORF mammalian expression plasmid, N-His tag||CG90116-NH|
|Rhesus IL10RB ORF mammalian expression plasmid, N-Myc tag||CG90116-NM|
|Rhesus IL10RB ORF mammalian expression plasmid, N-HA tag||CG90116-NY|
|Rhesus IL10RB natural ORF mammalian expression plasmid||CG90116-UT|
|Learn more about expression Vectors|
Interleukin 10 receptor, beta subunit (IL10RB/IL-10RB) also known as Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, and cytokine receptor family II, member 4, is a subunit for the interleukin-10 receptor. IL10RB/IL-10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. Defects in IL10RB/IL-10RB are the cause of inflammatory bowel disease type 25 (IBD25). It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.