|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MAP2K2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10678-ACG|
|Human MAP2K2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10678-ACR|
|Human MAP2K2 ORF mammalian expression plasmid, N-GFPSpark tag||HG10678-ANG|
|Human MAP2K2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10678-ANR|
|Human MAP2K2 ORF mammalian expression plasmid, C-Flag tag||HG10678-CF|
|Human MAP2K2 ORF mammalian expression plasmid, C-His tag||HG10678-CH|
|Human MAP2K2 ORF mammalian expression plasmid, C-Myc tag||HG10678-CM|
|Human MAP2K2 ORF mammalian expression plasmid, C-HA tag||HG10678-CY|
|Human MAP2K2 Gene cDNA clone plasmid||HG10678-M|
|Human MAP2K2 ORF mammalian expression plasmid, N-Flag tag||HG10678-NF|
|Human MAP2K2 ORF mammalian expression plasmid, N-His tag||HG10678-NH|
|Human MAP2K2 ORF mammalian expression plasmid, N-Myc tag||HG10678-NM|
|Human MAP2K2 ORF mammalian expression plasmid, N-HA tag||HG10678-NY|
|Human MAP2K2 natural ORF mammalian expression plasmid||HG10678-UT|
|Learn more about expression Vectors|
Dual specificity mitogen-activated protein kinase kinase 2, also known as MAP kinase kinase 2, MAPKK2, ERK activator kinase 2, MAPK / ERK kinase 2, MEK2 and MAP2K2, is a member of the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase subfamily. MAP2K2 / MEK2 contains one protein kinase domain. MEK1 and MEK2 (also known as MAP2K1 and MAP2K2, respectively) are evolutionarily conserved, dual-specificity kinases that mediate Erk1 and Erk2 activation during adhesion and growth factor signaling. MAP2K1 / MEK1 is a crucial modulator of Mek and Erk signaling and have potential implications for the role of MEK1 and MEK2 in tumorigenesis. MAP2K2 / MEK2 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. It also activates the ERK1 and ERK2 MAP kinases. Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) which is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy.