|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus TNFSF13 ORF mammalian expression plasmid, C-GFPSpark tag||CG90095-ACG|
|Rhesus TNFSF13 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90095-ACR|
|Rhesus TNFSF13 ORF mammalian expression plasmid, C-Flag tag||CG90095-CF|
|Rhesus TNFSF13 ORF mammalian expression plasmid, C-His tag||CG90095-CH|
|Rhesus TNFSF13 ORF mammalian expression plasmid, C-Myc tag||CG90095-CM|
|Rhesus TNFSF13 ORF mammalian expression plasmid, C-HA tag||CG90095-CY|
|Rhesus TNFSF13 Gene cDNA clone plasmid||CG90095-G|
|Rhesus TNFSF13 ORF mammalian expression plasmid, N-Flag tag||CG90095-NF|
|Rhesus TNFSF13 ORF mammalian expression plasmid, N-His tag||CG90095-NH|
|Rhesus TNFSF13 ORF mammalian expression plasmid, N-Myc tag||CG90095-NM|
|Rhesus TNFSF13 ORF mammalian expression plasmid, N-HA tag||CG90095-NY|
|Rhesus TNFSF13 natural ORF mammalian expression plasmid||CG90095-UT|
|Learn more about expression Vectors|
TNFSF13 is a member of the tumor necrosis factor (TNF) ligand family. It is a ligand for TNFRSF17/BCMA. TNFSF13 is lowly expressed in normal tissues, but is elevated in several types of tumors and transformed cell lines. It is mportant for B cell development. TNFSF13 may also play a role in T-independent type II antigen responses and T cell survival, and induce proliferation/survival of non lymphoid cells. It exists as a functional homotrimer. It can bind to two cell surface receptors, BCMA and TACI, which it shares with BAFF to exert downstream T- and B-cell regulatory effects. TNFSF13 also has been demonstrated to bind to proteoglycans on the cell surface.