|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MEK1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10661-ACG|
|Human MEK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10661-ACR|
|Human MEK1 ORF mammalian expression plasmid, N-GFPSpark tag||HG10661-ANG|
|Human MEK1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10661-ANR|
|Human MEK1 ORF mammalian expression plasmid, C-Flag tag||HG10661-CF|
|Human MEK1 ORF mammalian expression plasmid, C-His tag||HG10661-CH|
|Human MEK1 ORF mammalian expression plasmid, C-Myc tag||HG10661-CM|
|Human MEK1 ORF mammalian expression plasmid, C-HA tag||HG10661-CY|
|Human MEK1 Gene cDNA clone plasmid||HG10661-M|
|Human MEK1 natural ORF mammalian expression plasmid||HG10661-M-N|
|Human MEK1 ORF mammalian expression plasmid, N-Flag tag||HG10661-NF|
|Human MEK1 ORF mammalian expression plasmid, N-His tag||HG10661-NH|
|Human MEK1 ORF mammalian expression plasmid, N-Myc tag||HG10661-NM|
|Human MEK1 ORF mammalian expression plasmid, N-HA tag||HG10661-NY|
|Human MEK1 natural ORF mammalian expression plasmid||HG10661-UT|
|Learn more about expression Vectors|
MEK1, also known as MAP2K1 and MKK1, is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Binding extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause cardiofaciocutaneous syndrome.