|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus IGF1R ORF mammalian expression plasmid, C-GFPSpark tag||CG90082-ACG|
|Rhesus IGF1R ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90082-ACR|
|Rhesus IGF1R ORF mammalian expression plasmid, C-Flag tag||CG90082-CF|
|Rhesus IGF1R ORF mammalian expression plasmid, C-His tag||CG90082-CH|
|Rhesus IGF1R ORF mammalian expression plasmid, C-Myc tag||CG90082-CM|
|Rhesus IGF1R ORF mammalian expression plasmid, C-HA tag||CG90082-CY|
|Rhesus IGF1R Gene cDNA clone plasmid||CG90082-G|
|Rhesus IGF1R ORF mammalian expression plasmid, N-Flag tag||CG90082-NF|
|Rhesus IGF1R ORF mammalian expression plasmid, N-His tag||CG90082-NH|
|Rhesus IGF1R ORF mammalian expression plasmid, N-Myc tag||CG90082-NM|
|Rhesus IGF1R ORF mammalian expression plasmid, N-HA tag||CG90082-NY|
|Rhesus IGF1R natural ORF mammalian expression plasmid||CG90082-UT|
|Learn more about expression Vectors|
The insulin-like growth factor-1 receptor (IGF1R) is a transmembrane tyrosine kinase involved in several biological processes including cell proliferation, differentiation, DNA repair, and cell survival. This a disulfide-linked heterotetrameric transmembrane protein consisting of two α and two β subunits, and among which, the α subunit is extracellular while the β subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF1R signalling pathway is activated in the mammalian nervous system from early developmental stages. Its major effect on developing neural cells is to promote their growth and survival. This pathway can integrate its action with signalling pathways of growth and morphogenetic factors that induce cell fate specification and selective expansion of specified neural cell subsets. Modulation of cell migration is another possible role that IGF1R activation may play in neurogenesis. In the mature brain, IGF-I binding sites have been found in different regions of the brain, and multiple reports confirmed a strong neuroprotective action of the IGF-IR against different pro-apoptotic insults. IGF1R is an important signaling molecule in cancer cells and plays an essential role in the establishment and maintenance of the transformed phenotype. Inhibition of IGF1R signaling thus appears to be a promising strategy to interfere with the growth and survival of cancer cells. IGF1R is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target.