|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus TGFBR2 ORF mammalian expression plasmid, C-GFPSpark tag||CG90063-ACG|
|Rhesus TGFBR2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90063-ACR|
|Rhesus TGFBR2 ORF mammalian expression plasmid, C-Flag tag||CG90063-CF|
|Rhesus TGFBR2 ORF mammalian expression plasmid, C-His tag||CG90063-CH|
|Rhesus TGFBR2 ORF mammalian expression plasmid, C-Myc tag||CG90063-CM|
|Rhesus TGFBR2 ORF mammalian expression plasmid, C-HA tag||CG90063-CY|
|Rhesus TGFBR2 Gene cDNA clone plasmid||CG90063-G|
|Rhesus TGFBR2 ORF mammalian expression plasmid, N-Flag tag||CG90063-NF|
|Rhesus TGFBR2 ORF mammalian expression plasmid, N-His tag||CG90063-NH|
|Rhesus TGFBR2 ORF mammalian expression plasmid, N-Myc tag||CG90063-NM|
|Rhesus TGFBR2 ORF mammalian expression plasmid, N-HA tag||CG90063-NY|
|Rhesus TGFBR2 natural ORF mammalian expression plasmid||CG90063-UT|
|Learn more about expression Vectors|
TGFBR2 is member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. It is a transmembrane protein. TGFBR2 is comprised by a C-terminal protein kinase domain and an N-terminal ectodomain. The ectodomain consists of a compact fold containing nine beta-strands and a single helix stabilised by a network of six intra strand disulphide bonds. The folding topology includes a central five-stranded antiparallel beta-sheet, eight-residues long at its centre, covered by a second layer consisting of two segments of two-stranded antiparallel beta-sheets. TGFBR2 has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is increased in oral squamous cell carcinoma cells. Its expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity.